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KMID : 0350519940470041613
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Journal of Catholic Medical College
1994 Volume.47 No. 4 p.1613 ~ p.1626
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Relation between p53 Protein Expression and ras p21 Protein Expression, p53 Gene Point Mutation, 17p Allelic Deletion in Colorectal Adencarcinomas
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Abstract
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ras gene mutation, point mutation of p53 tumor suppressor gene, and 17p allelic deletion, leading to the progressive disordering of the normal growth controlling mechanisms in colorectal cancers. Among those genetic alterations, ras gene point
mutation
and p21 protein expression are thought to be a event of the benign and early malignant phase. And p53 gene point mutation and 17p allelic deletion are believed to be early or middle event of malignant tumor growth. Recently authors observed that
higher
positive expression of p53 protein in differentiated, early Dukes' stage , no invasion of lymphatics and perineural tissues of colorectal cancers.
In order to investigate the relation between p53 protein expression and ras p21 protein expression, p53 gene point mutation, 17p allelic deletion, authors selected 40 cases among 70 cases of p53 protein stained with mouse anti-p53 protein
monoclonal
antibody, DO7 (wild and mutant form), divided experimental group into p53 protein positive group(group 1, 20 cases) and p53 protein negative group(group 2, 20 cases). And immunohistochemical staning with mouse anti-p21 protein pan-ras monoclonal
antibody, detection of point mutation on codon 175 of p53 gene, and detection of 17pallelic deletion by p YNZ22.1 and p144-D6 probes were done in each group. The incidence and significancy of p21 ptoein expression, p53 gene point mutation and 17p
allelic deletion was compared in each group. Results obtained were as follows:
1. Expression of p53 protein in 70 cases of colorectal adenocarcinomas was positive in 41.4% of cases.
2. Positive expression of p21 protein in p53 protein positive group(85%) was much higher than in p53 protein negative group(65%) and also the degree of expression was higher in p53 protein positive group than negative group.
3. 17p allelic deletion by pYNZ22.1 probe was similar expression in both group(80% and 85%). The deletion by pYNZ22.1 in 40 cases of both group was in 82.5%(33/40).
Allelic deletion by p144-D6 probe revealed also similar to pYNZ22.1 probe in both group(80% and 85%). 17p deletion with both probes was in 67.5% of total cases of both group.
4. p21 protein expression was higher positive p53 protein group than negative group. But 17p allelic deletion was revealed no relation with p53 protein expression. Point mutation of codon 175 of p53 gene was in 30% of 40 cases in colorectal
cancer
without relation of p53 protein expression.
With above results, authors insist that p53 protein and p21 protein expression are related with higher expression in early stage of malignant tumor growth, but point mutation and 17p allelic deletion are not related with positive p53 protein
expression.
And so we consider the p53 protein expression and p21 protein expression are available in early detection of colorectal cancers.
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